In-silico functional annotation of a hypothetical protein from Edwardsiella tarda revealed Proline metabolism and apoptosis in fish

Edwardsiella tarda is one of the most widespread pathogens in aquatic species. A wide variety diseases can be caused by this microbe, including septicaemia but clinical signs infection differ between species fish. The fact that bacteria resistant to a range antimicrobials extremely important. Furthermore, several proteins its genome are classified as hypothetical (HPs). As result, current work sought elucidate roles HP found E.tarda. To determine structure and function protein, many bioinformatics methods were used. locate homologous sequence similarity was searched across available databases. Quality evaluation used predict confirm secondary tertiary structure. Additionally, active site interacting examined using CASTp STRING server. An important biological activity it contains single functional domains may responsible for host-cell invasion autolysis. Further, protein-protein interactions within selected revealed partners essential bacterial survival. One such partner proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (putA) E. tarda. In addition, molecular docking simulation results showed stable bonding Proline metabolism protein. Finally, shows annotated associated with possible mitochondrial autolysis formation activities, well having binding putA which might significant relevance future genetics research.